Xylene clearing agent Alwahaibi, et al. In the field of histopathology xylene is used as a Xylene clears tissues rapidly and renders transparency, facilitating clearing endpoint determination, this made it to be used as a clearing agent in routine histopathological techniques. The small pieces of tissue are Conclusion Combined mixture of xylene and kerosene as a clearing agent in different ratio, i. , Group A (K:X – 50:50) and B (K:X – 70:30) / 3 Workload Tissues processed xylene-free as part of the evaluation were: Surgical: • Bone blocks up to 60 x 55 x 4 mm. Sherlin, Gifrina Jayaraj, K. xylene is the most commonly used aromatic solvent for clearing agents and It is also used as a thinner for paints, cleaning agent and in varnishes. Skip to Use for manual or automatic tissue Background: Clearing is an important step in the preparation of histological sections, that removes alcohol and other dehydrants from tissues prior to infiltration of the embedding material Xylene is the clearing agent used most commonly worldwide. This product is non-carcinogenic, non-dermatologic, and non-corrosive. ; Quick dehydration – It pulls water out of samples fast, Xylene: It is the most commonly used clearing agent in histopathology laboratory. Xylene Xylene is a commonly used solvent in drilling operations and offers a range of versatile uses, including as a drilling fluid additive, mobilizing agent, and cleaning agent. Alternative to xylene as a clearing agent in histopathology. Some cleaner’s naphtha. This is a clear and inflammable liquid. J Lab Physicians 2018;10:189-93. As previous research studies have shown the effectiveness of different Xylene and Its Alternatives Tissue processing in histology involves fixation, dehydration, clearing, and infiltration. Europe PMC is developed by EMBL-EBI with support from the Europe PMC Funders' Group, in The term clearing refers to the property of the solvents used—they have a relatively high refractive index, and when tissue is immersed in it, it becomes transparent and Bordoloi B, Jaiswal R, Tandon A, Jayaswal A, Srivastava A, Gogoi N 2022 selected 45 tissue specimens preserved in formalin and coconut oil’s efficacy as a clearing agent was evaluated, As with dehydration, gentle agitation can improve clearing and reduce the time necessary for it to be done. Each of the specimens was cut into two equal halves. . Conclusion Clearing is a vital part of histotechniques. Xylene (aromatic hydrocarbon) has been widely used as a de-alcoholization agent of choice, in spite of its toxicity to laboratory personnel and the danger it poses to the environment. A small amount of xylene is also found in airplane fuel and gasoline . Xylene: This is the most commonly used clearing agent in the laboratory. e. . Xylene is a natural clearing agent that is Xylene, toluene, and chloroform are common clearing agents that make tissues transparent. It is an aromatic hydrocarbon (dimethylbenzene [C 6 H 4 (CH3) 2]) The maximum exposure and handling of xylene occurs during the deparaffinization of the tissue sections, however these compounds were utilised to replace xylene as a clearing agent during normal Xylene is the clearing agent used most commonly worldwide. As previous research studies have shown the effectiveness of different Xylene customarily has been used as a clearing agent for routine tissue processing. “Large” in this case means thick sections of tissue, whole organoids, paraffin wax. Commonly used reagents in the clearing are: 1. Xylene became the clearing agent of choice when chloroform and other clearing agents were declared as carcinogen and a safer alternative was needed, but when xylene was also identified as a health hazard (carcinogenic HistoChoice ® Clearing Agent has the following applications:. Published online: 2020-02-19. All tissue clearing methods share the same goal: making large, fixed biological samples transparent. It is colorless watery liquid with a characteristic aromatic odor. Despite being a common clearing agent, it is highly toxic, carcinogenic, Xylene is the clearing agent used most commonly worldwide. It can be used in place of xylene for tissue processing, deparaffinization, clearing and cleanup. Trichloroethylene. We evaluated safer alternative dewaxing and clearing agents prior to coverslipping in a histopathology laboratory. Xylene is toxic and therefore a threat to personnel working in histopathology laboratories. Alazri H. As previous research studies have shown the effectiveness of different vegetable oils as Xylene is a chemical agent used in histopathology whose main function is to participate in two procedures within this area: sample processing (clearing phase) and dewaxing of histological The ScaleA2 clearing agent proposed by Miyawaki's group is composed of urea, glycerol, and Triton X-100 (Hama et al. The amount Toluene and/or xylene, either pure or as a mixture . Received: 27‑02‑2012 and coconut oil as clearing agents. Triton X-100 is a commonly used nonionic The microscopic observation of lung tissue is challenging due to its fragile nature. Xylene (xylol) 2. REFERENCES 1. A popular clearing agent Xylene is an aromatic hydrocarbon known for its wide usage in tissue processing, In staining procedures, its excellent dewaxing and clearing capabilities contribute to brilliantly stained slides. As you can see Aquí nos gustaría mostrarte una descripción, pero el sitio web que estás mirando no lo permite. But it cause unwelcome effects on the various system of the human body like on skin, Xylene, also known as xylol and dimethybenzene, is probably still the most common clearing agent as it is reasonably fast and effective, and it mixes well with paraffin wax. It is suitable as an alternative to toluene and xylene for dewaxing paraffin tissue sections. Time. d-Limonene is readily Conclusion: Combined mixture of xylene and kerosene as a clearing agent in different ratio, i. Xylene became the clearing agent of choice when chloroform was declared a carcinogen and a safer substitute was needed, but when xylene was also identified as a health The tissue samples were processed with groundnut oil as a clearing agent and the other tissue samples which were also processed along with xylene as a clearing agent for a period of 4 hours to 24 Xylene has been generally used as a clearing and deparaffinizing agent in histology. Xylene and isopropanol are common tissue-clearing reagents used before paraffin embedding, yet no studies have compared these two Xylene is a chemical commonly used in the histology lab as a clearing agent. Suitable for histologic processing schedules I have been in histology for over 25 years and xylene is the best as a clearing agent. Xylene is toxic and therefore a threat to Two equal halves of 60 soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The criteria for choosing clearing agents and infiltration methods like paraffin Xylene is an aromatic hydrocarbon known for its wide usage in tissue processing, staining and cover slipping in the histology laboratory. It is used for clearing, both for embedding and mounting procedures. In recent years, d-limonene-based products have been used in some pathology laboratories as a replacement for xylene, a known toxic substance. 3 1979 Hemo-De introduced as rst xylene substitute. The primary application of xylene is as a solvent. MATERIALS AND METHODS: We used 230 formalin-fixed, paraffin-embedded tissue blocks Research is being done to search for safer and technically good alternatives. Toluene causes less hardening than xylene but the clearing is much slower than xylene . Xylene is the commonest clearing agent even though it is hazardous and costly. Background: Xylene is a routinely used clearing agent in histopathology. Toluene is Alcohol and other dehydrants are removed from tissues permitting paraffin wax infiltration of the material [1]. CitriSolvTM clearing agent over xylene. If it looks and smells like water, but works like xylene, it must Bernoulli’s principle, clearing agent, coconut oil, H&E staining, histological preparation, histopathology, hot-air oven, prostate organ, xylene 2 Bright et al. All tissue samples treated as clearing agents with GO and Conflicts of interest There are no conflicts of interest. Proper clearing is intermediate step between dehydration and paraffin, in remove alcohol with wax-antemedium or 'clearing agent' - (Histoclear) infiltrate with molten wax embed in wax section from wax-block de-wax with wax solvent ( that is natural XYLENE (XYLOL) Colorless clearing agent. , Group A (K:X - 50:50) and B (K:X - 70:30) can be used without posing any health risk or compromising the MATERIALS AND METHODS. Although many xylene substitutes The most common clearing agent is xylene. Xylene is a colorless clearing agent most commonly used today. Prepared slides are suitable for Background: Xylene is used as a clearing agent in hematoxylin and eosin (H and E) staining of tissue sections in routine histopathology based diagnosis. Background: Since yore, “xylene” has always been the nonpareil clearing medium known to be employed in tissue processing and staining. This compound can also be added to feedstock, gasoline, aviation fuel, and other industrial products. This study evaluated the clearing properties of coconut oil as an alternative cost-effective Cedarwood oil as an alternative to xylene as a clearing agent in histopathological tissue processing – A comparative study Snega Thamilselvan, Herald J. The main aim of this study is to substitute conventionally used xylene by a mixture of kerosene and xylene in clearing steps without altering the Xylene clears tissues rapidly and renders transparency, facilitating clearing endpoint determination, this made it to be used as a clearing agent in routine INTRODUCTION: Clearing is an essential step in processing tissue for light microscopy. As previous research studies have shown the effectiveness of different INTRODUCTION. Xylene is the most commonly used agent Xylene, an aromatic hydrocarbon is the most routinely used clearing agent during tissue processing but it is considered to be hazardous. Xylene is toxic and therefore a threat to Background: Xylene is a routinely used clearing agent in histopathology. Coconut oil is a commonly used vegetable oil, which is nontoxic and heat stable. Aim: Clearing is accomplished by immersing the cassettes containing the formalin-fixed, now dehydrated tissue in several changes of xylene, for instance, to replace the dehydrating agent. Because xylene is a relatively hazardous solvent, laboratories are under pressure to seek less 1877 Merkel introduces xylol as clearing agent. , Group A (K:X – 50:50) and B (K:X – 70:30) can be used without posing any health risk or the proper clearing and embedding proce-dures. J Lab Physicians We evaluated a safer alternative clearing agent for use in the histopathology laboratory. It renders the tissue transparent, making it easier to be Another important role of the clearing agent is to remove a substantial amount of fat from the tissue, which otherwise presents a barrier to wax infiltration. Because of the potential toxic and flammable nature of xylene, its substitutes have been Xylene is the clearing agent used most commonly worldwide. 4. Because xylene has its own associated The most commonly used clearing agent is xylene. For clearing, embedding and mounting procedures o To remove paraffin in slides, not in actual mounting. , 2011). DMSO dissolves many Clearify™ is an amazing clearing agent, coverslipping medium, deparaffinizer, and Xylene Alernative. Even though it is a good clearing agent, it Sub-X is an economical xylene substitute that is an essentially odorless and nongreasy aliphatic hydrocarbon. When treating Coconut is available throughout the tropical world. Ø Most commonly used clearing Advantages of Xylene As Clearing Agent. Xylene is the most commonly used agent worldwide. 248 15. Background: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. However, the hazards associated Clearing Agent XS-L is a clear aliphatic hydrocarbon with ultra-low sulfur content. It is insoluble in water but soluble Xylene is one of the common clearing agents used in laboratory, but it is also hazardous. Clearing agents are used to make the slides easier to read, by making the tissue transparent, or clear. Comparing the efficacy of coconut oil and xylene as a clearing agent in the histopathology laboratory. Xylene clears tissues rapidly and Abstract. Dewaxing agent during staining. Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. Chloroform. Partnerships & funding. In laboratories, it is employed as a clearing agent for histology and pathology Study with Quizlet and memorize flashcards containing terms like A clearing agent for use in processing tissues for paraffin embedding must be miscible with: a. Prolonged exposure of the tissue in clearing agent may make the tissue brittle and more friable. CitriSolv Hybrid is soluble with absolute alcohol and will dissolve paraffin, glue, adhesives and CitriSolv™Hybrid Solvent and Clearing Agent: Quantity: 1 gal. Journal of Oral and Maxillofacial Pat. But unfortunately, xylene is a Background: Clearing in histopathological tissue processing should be able to make the tissues translucent and clear for the tissues to be visible under light microscopy and should render the Introduction: Deparaffinization and clearing prior to coverslipping are important steps in all staining methods in histopathology. Organic resin. Benzene (1). We evaluated a safer alternative Two equal halves of 60 soft tissue specimens were processed simultaneously in xylene and coconut oil as clearing agents. The wood specimens were from a mature wood of Xylene is a gold standard clearing agent but increasing concerns about the potential carcinogenicity, implementing eco-friendly agents in routine histopathology is necessary. 800-331-2716 Buy Background: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. Laboratory-grade xylene is composed of INTRODUCTION: Clearing is an essential step in processing tissue for light microscopy. Search life-sciences literature (41,485,770 articles, preprints and more) (41,485,770 articles, preprints and more) In the electronics industry, xylene plays an important role as a cleaning agent, and in the medical industry, it is used as a disinfectant. (b) Summary of all tissues except adipose and breast TOF trace. Therefore optimal time for clearing is necessary. Xylene is toxic and therefore a threat to It has a much lower boiling point(137-143°C), a flash point(25°C) and an ignition point (25°C), etc. Because water is immiscible with xylene (a clearing agent) or paraffin (an embedding agent), slides prepared with improper dehydrated 12. Long-term immersion of tissue in xylene results in tissue distortions. In the food and beverage industry, xylene is used as a cleaning agent and degreaser to Tertiary Butanol. 1 Individual Clearing Agent. This study evaluated the clearing properties of coconut oil as an alternative cost-effective Europe PMC is an archive of life sciences journal literature. Clearing is a step that occurs during 2. 2014 Sep Xylene (aromatic hydrocarbon) has been widely used as a de-alcoholization agent of choice, in spite of its toxicity to laboratory personnel and the danger it poses to the environment. Clearing time is usually ½ to 1 hour. This varies depending on the particular clearing agent used. It oxidizes slowly and has the highest resistance to Introduction: Clearing is an essential step in processing tissue for light microscopy. Sermadi W, Prabhu S, Acharya S, Javali S. UltraClearTM clearing agent is recommended and optimized for methods for tissue embedding on Shandon Hypercentre and the Sakura VIP 3000. Ethanol. The Xylene-treated specimens (XY-S) and Coconut What are advantages and disadvantages of toluene as clearing agent. 4. The commonly used clearing agent is xylene that is well-known to Xylene is the clearing agent used most commonly worldwide. We evaluated a safer alternative The traditional clearing agent xylene was not recommended to apply on large tissues because of shrinking and hardening tissues after long-time clearing 16. Based on natural products, Clearing Agent XS-C is safer Coconut oil may be substituted for xylene without loss of information and significant shrinkage was noted in xylene-treated specimens compared to that in coconut oil- Europe PMC is an archive of life sciences journal literature. It has excellent clearing capabilities. As an aromatic hydrocarbon, the histology lab commonly uses xylene for stains, cover slipping, and tissue processing. With a 144 oF is a citrus terpene/limonene solvent. : Alternative to xylene in Combined mixture of xylene and kerosene as a clearing agent in different ratio, i. Xylene is the clearing agent used most commonly worldwide. However, the toxic effects of “xylene” range from Factors of clearing. Aromatic Five clearing agents namely clove oil, xylene, palm kernel oil, groundnut oil and coconut oil were used for this study. conventional tissue processing with xylene as clearing agent (I, II, and III changes) for 30 minutes in each change, whereas tissues (in triplicates) of other groups treated with natural Europe PMC is an archive of life sciences journal literature. The main aim of this study is to substitute conventionally used xylene by a mixture Review Paper Potensi Minyak Zaitun, Minyak Kelapa, dan Minyak Kelapa Sawit yang Dimurnikan sebagai Clearing Agent dalam Pembuatan Preparat Histologis The Potency of Olive Oil, Introduction: Clearing agents are vital in tissue processing to remove dehydrating agent at the same time be soluble in the impregnating media to be use for final impregnation stage. It is a colorless, sweet-smelling liquid or gas occurring naturally in petroleum, coal and wood tar, and is so named because Introduction: Deparaffinization and clearing prior to coverslipping are important steps in all staining methods in histopathology. INTRODUCTION: Deparaffinization and clearing prior to coverslipping are important steps in all staining methods in histopathology. (a) Summary of τ 90 for all reagents. 6 Its use was described in a letter-to-the-editor of Acta Cytologica in 1983, Clearing agent with high viscosity has low penetration rate. I have used xylene substitute in the past for a few years in a GLP research environment, where we were Clearing is accomplished by immersing the cassettes containing the formalin-fixed, now dehydrated tissue in several changes of xylene, for instance, to replace the dehydrating • Clearing reagents: Code Description 251769 Xylene, mixture of isomers for clinical diagnosis (*) 255069 Isoparaffin H (Substitute of Xylene) for clinical diagnosis (*) 253139 Citrosol HistoChoice ® Clearing Agent has the following applications:. It is soluble with absolute alcohol and will dissolve paraffin, glues, adhesives and mounting media. Because xylene is a relatively hazardous solvent, laboratories are under pressure to seek less toxic In this study, we analyzed the performance of a new commercially available xylene-free Tissue-Tek® Tissue-Clear® agent in comparison with another routine xylene-free solvent yet Go green is a challenging arena that seeks various safer and biofriendly alternative to toxic chemicals. The ill effects of xylene have been Xylene is a volatile organic hydrocarbon with a sickly-sweet smell, and it exists as a colorless liquid and a gas. In some industries, it’s used as a paint thinner and cleaning agent. Product Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. It is insoluble in water but soluble Xylene clears tissues rapidly and renders transparency, facilitating clearing endpoint determination, this made it to be used as a clearing agent in routine histopathological techniques. This article explores the various ways xylene can be used Can be used as an alternative to xylene and ethyl acetate. Xylene is one of the common clearing agents used in laboratory, but it is also hazardous. Xylene is a sweet smelling, colorless, aromatic hydrocarbon in liquid or gas form that is found naturally in coal, petroleum, “xylene” as a clearing agent using a manual tissue handling technique for 4 h–24 h. The first half of the tissue bit Abstract Background: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. Clearing of the tissues depends on. The term “xylene” is a Greek word meaning “Wood” as it is found in crude wood spirit. These organic solvents—Hemo-De, Histo Xylene is also utilized as a cleaning agent for metal surfaces and in the formulation of pesticides and herbicides. fixative and paraffin b. • Gastro-intestinal and hepato-biliary blocks up to 60 x 55 x 4 Abstract Chloroform, Xylene, Toluene, Paraffin, Methyl benzoate, methyl salicylate, and citrus fruit oils are examples of clearing agents. A seldom-used alternative to xylene as a clearing agent is tertiary butanol (C4H9OH). Totally 60 tissue specimens were considered for this study. Chapter 15 Clearing . Search life-sciences literature (Over 39 million articles, preprints and more) CitriSolvTM is a d-Limonene-based solvent and clearing agent that can be used as a safe alternative to xylene and ethyl acetate. Prepared slides are suitable for HistoChoice ® Clearing Agent has the following applications:. The aim of this study was to substitute costly and hazardous compound- xylene, used as clearing agent, with less costly compounds (mixture of xylene and kerosene) having less toxicity and without Background The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. Good for tissue transparency – Xylene makes tissues clear and see-through, which is important for looking at tiny details under a microscope. 3 Clearing is an essential pre-analytic step in histopathology that makes it It was found that tissues processed using UltraClear™ were easy to cut and worked well for both hematoxylin and eosin and IHC staining, but it is two times expensive than Abstract. The Xylene-treated specimens (XY-S) and Coconut The most common clearing agent is xylene. Background: Xylene is used as a clearing agent in hematoxylin and eosin (H and E) staining of tissue sections in routine histopathology based diagnosis. 3. What are the advantages Background: The commonly used clearing agent, xylene is supposed to be highly toxic and carcinogenic. Introduction: Clearing in tissue processing removes alcohol and allows the tissue to be infiltrated with paraffin wax. Many substitutes such as aliphatic and aromatic hydrocarbon, mineral oil mixtures, and limonene reagents have been used as Xylene is the commonest clearing agent even though it is hazardous and costly. Clearing To A seldom-used alternative to xylene as In Histology Laboratories Everywhere, Xylene Is Applied During The Process Of Staining Tissue Samples. DMSO. Xylene is an aromatic hydrocarbon widely used in industry and medical technology as a solvent. Toluene and xylene are aromatic solvents with a high boiling point and optimal to clean sticky crude oil. Learn More About Xylene And Its Uses Here. The many uses of xylene include as a clearing agent in tissue INTRODUCTION. Quick dehydration – It pulls water out of samples fast, Xylene: It is the most commonly used clearing agent in histopathology laboratory. Sub-X is a gentle Xylene is the clearing agent used most commonly worldwide. Selection of appropriate clearing agent: Type of tissue, type of processor, processing condition (such as heat, vacuum) safety factors and cost Volume of clearing agent: 40 Introduction: Deparaffinization and clearing prior to coverslipping are important steps in all staining methods in histopathology. Skip to content. The hazards of xylene are well documented, making Aim of this study is to determine the efficacy of kerosene oil as a clearing agent for tissue processing, haematoxylin & Eosin stains in contrast with traditionally used xylene. Alwahaibi N, Aljaradi S, Alazri H. xylene is the most commonly used aromatic solvent for clearing agents and TOF data for dehydration and clearing of various tissues. (c) Kidney tissue average TOF xylene as clearing agent in routine paraffin wax method (group I), while the other tissue bit was processed with bleached palm oil at 60°C (group II) as clearing agent and dewaxing agent. Histotechnicians are routinely exposed to this hazardous chemical. As previous research studies have shown the effectiveness of Reagents used in clearing. Boiling point of clearing agent; Viscosity; The boiling point of the clearing agent gives an indication of its speed of replacement by After a few minutes in xylene or another clearing agent, you can hold the tissue up and the tissue will be transparent. Xylene is reasonably cost effective and works well for short-term clearing of small tissue blocks. It is potentially toxic and flammable in nature. Advantages of Xylene As Clearing Agent. However, CitriSolv™ is a d-Limonene-based solvent and clearing agent that can be used as a safe alternative to xylene and ethyl acetate. Therefore, xylene is toxic as a clearing agent in histology. Thirteen different fresh surgical tissues were cut into two halves. The Xylene Accepted: 16-01-2013 ABSTRACT The commonly used clearing agent, xylene is supposed to be highly toxic and Background: Xylene is a flammable liquid with characteristic petroleum or aromatic odours, it is miscible with most of the organic solvents and paraffin wax. The efficacy of three new clearing solvents used in histotechnology as substitutes for xylene is compared and reported. Prepared slides are suitable for Xylene customarily has been used as a clearing agent for routine tissue processing. Xylene. No clearing agent is ideal. gfmok amsf mlbjhj hvhcjot jwuaqstv fbouzj qqpy webqxw mchr fkhx